Analysis of the relativity between the drug resistance and genotype of clinical strains isolated from tuberculous meningitis

doi:10.3969/j.issn.1000-6621.2014.06.002

Abstract

Abstract: Objective To study the relativity between the drug resistance and genotype of clinical strains isolated from tuberculous meningitis(TBM). Methods M. tuberculosis was cultured and isolated in cerebrospinal fluids (CSF) from 25 cases diagnosed with TBM in Xijing Hospital. These isolates were performed the susceptibility testing of 14 antituberculosis drugs, the sequencing of 10 drug-resistant genes and strain typing by Spoligotyping. Results Of 25 M. tuberculosis isolates, 20 (80.0%) clinical isolates were identified as Beijing genotype by Spoligotyping; 3 (3/25) strains were multidrug-resistant (MDR) and belonged to Beijing genotype, in which katG, rpoB, gyrA, rrs-KAN, rpsL, gidB genes were found the mutations, but inhA, embB, gyrB, eis genes had not mutation by the DNA sequencing. Conclusion Beijing genotype strains may act as the main epidemic strain of TBM，especially MDR-TBM.

Key words: Tuberculosis, meningeal, Mycobacterium tuberculosis, Drug resistance, bacterial, Genotype

WANG Ting, ZHAO Yan-lin, PANG Yu, ZHOU Yang, FENG Guo-dong, PENG Jin-xiang, ZHAO Gang. Analysis of the relativity between the drug resistance and genotype of clinical strains isolated from tuberculous meningitis[J]. Chinese Journal of Antituberculosis, 2014, 36(6): 411-417. doi: 10.3969/j.issn.1000-6621.2014.06.002

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